Changes of Recognition to Death Before and After Observation on the Cadaver Dissection to Paramedical Students / 체질인류학회지

The aim of this study is to identify changes of recognition to death before and after observation on the cadaver dissection to paramedical students. This study was done on 472 freshmen using questionnaire survey. Recognition to death questionnaire was consisted of 36 items. Data were collected before and after observation on the cadaver dissection with agreement of subjects. The data were analyzed using SPSS win 24.0. This study showed that recognition to death was significantly increased after observation on the cadaver dissection (3.19 points) than before observation on the cadaver dissection (3.06 points) (p=.000). Especially, anxiety on death was significantly increased (p=.000), and interest in death was significantly increased, too (p=.000). The results of this study suggest that we need positively to encourage observation on the cadaver dissection for paramedical students with providing program to decrease anxiety on death because of not only improving anatomy knowledge but also increasing recognition to death.

Utilization of Second Digit to Fourth Digit Ratio (2D : 4D) as One of Physical Markers to Evaluate Aggression in Elementary School Students / 체질인류학회지

The aim of this study is to provide basic data to prevent adolescent crime or violence by analyzing aggression according to second digit to fourth digit ratio (2D : 4D). Index and ring finger length on both hands by photocopy was measured using Callipers from ventral proximal crease to finger tip. This study was done on 187 elementary school students (98 males, 89 females). Data were collected by measuring index and ring finger length on both hands by photocopy and by completing self-reported questionnaire. The data were analyzed using SPSS win 21.0. This study showed that the 2D : 4D of males was significantly lower than female, and the aggression score of males was significantly higher than female. Especially, there was significantly differences between 0.900 or less than 0.900 digit ratio group and 1.000 or more than 1.000 digit ratio group only left hand of males. The results of this study suggest that left digit ratio of males in elementary school students are able to be used as one of physical markers to evaluate aggression.

Change of Second to Fourth Digit Ratio according to Age in Korean Children / 체질인류학회지

The 2nd to 4th digit ratio (2D : 4D) reflects exposure level of sex hormones in fetal period. The aim of this study is to identify a change of digit ratio according to age and a critical age showing gender difference in Korean children. This study was done on 1,182 children (588 males, 594 females). Data were collected by measuring index and ring finger length on both hands by photocopy. The data were analyzed using SPSS win 21.0. This study showed that the 2nd and 4th finger length and digit ratio of both hands increased with age both male and female. There was no differences in 2nd to 4th digit ratio between males and females until they were 3 years old. However, there were significant differences in 2nd to 4th digit ratio between males and females from the age of four. The results suggest that digit ratio in Korean children increases with age and a critical age showing gender difference is from the age of four.

Difference of Second to Fourth Digit Ratio according to the Methods of Measuring Length / 체질인류학회지

The 2nd to 4th digit ratio (2D : 4D) reflects exposure level of sex hormones in fetal period and there are various methods for finger length measurement. The aim of this study is to identify an accurate and effective method for finger length measurement. This study was done on 272 individuals (115 males, 157 females). Data were collected by measuring index and ring finger length on both hands by calipers, ruler, photocopy, and radiography. The data were analyzed through independent t-test, paired t-test, Pearson correlation analysis using SPSS win 19.0. This study showed that the 2nd and 4th finger measured by radiography were the longest finger both of males and females and revealed the largest length difference between 2nd and 4th finger. There were significant sex differences all of 4 measurement methods in 2D : 4D, especially remarkable sex difference by radiography and photography. The 2D : 4D by indirect measurement was lower than that of direct measurement. Correlation between digit ratio by radiography and by photocopy was 0.590 and correlation between digit ratio by radiography and by calipers was 0.586. The results of this study suggest that indirect measurement by radiography can be alternated by photocopy primarily and by calipers secondarily.

Podocyte-specific Expression of C1q/TNF-alpha Related Protein 1 in Mice / 체질인류학회지

C1q/TNF-alpha Related Protein 1 (CTRP1), an adiponectin paralog, is a novel member of the C1q-TNF Related Protein family. CTRP1 is expressed in the kidney, although its localization and role in the kidney have not been studied. This study examined CTRP1 expression and function in the kidney. CTRP1 immunohistochemistry and PAS staining of the kidneys of C57/BL6 and FGS/Nga mice were performed. In situ hybridization for podocin in the kidney was also performed. CTRP1 immunoreactivity was found only in the glomeruli of the kidney. The CTRP1-immunoreactive cells in the glomeruli were identified as podocytes. The number of CTPR1-immunoreactive cells and the intensity of CTRP1 immunoreactivity were lower in the glomeruli of FGS/Nga mice, which develop progressive proteinuria and focal glomerulosclerosis. CTRP1 is a novel protein expressed in podocytes of the mouse kidney and may have a role in podocytes related to glomerular filtration in the kidney.

Second to Fourth Digit Ratio as a Sex Determinant in Korean / 체질인류학회지

The 2nd to 4th digit ratio (2D : 4D) as a predictor of the degree of expression of sexually dimorphic and other sex-hormone-mediated traits differs between the sexes, ethnics and race. The aim of this study is to identify characteristics and role of 2nd to 4th digit ratio as a sex determinant in Korean. This study was done on 664 individuals (332 males, 332 females). Data were collected by measuring index and ring finger length on both hands with digital callipers. The data were analyzed through the frequency, descriptive statistics, X2 test, independent t-test, paired t-test, Pearson correlation analysis using SPSS win 19.0. This study showed that the 2nd to 4th digit ratio (2D : 4D) in Korean men (0.96) was significantly lower than that of women (0.97). There was a significant difference in the types of 2nd and 4th finger length according to sex. There was a significant difference between right 2D : 4D and left 2D : 4D both of Korean men and women. Correlation between right and left hand digit ratio was .530 in men and .556 in women. The 2nd to 4th digit ratio accurately determinated sex in 89.1% males and 89.2% females for the left hand, and in 91.8% males and 91.9% females for the right hand. The results of this study suggest that 2nd to 4th digit ratio in Korean differs between males and females, moreover, it will be able to perform a role as a sex determinant.

Influencing Anthropometric Values to Atd Angles on Palm Prints in European American / 체질인류학회지

American population consists of people of various race and ethnic group. Palm prints are different from each individual, ethnic group and race. The aim of this study is to investigate influencing anthropometric values to atd angles formed by triradii of palm prints in European American and develop a regression equation that can predict atd angle. This study was done on 208 European American (males: 102 individuals, females: 106 individuals). Data were collected by measuring atd angles using a protractor, distance from triradii a to d, length from triradius t to the distal wrist crease (DWC), palm breadth at metacarpals and palm length perpendicular using digital callipers. The data were analyzed by descriptive statistics, the frequency, independent sample t-test, paired t-test, Pearson correlation analysis, stepwise regression using SPSS win 18.0. This study showed that atd angles of males (38.6degrees) are significantly smaller than those of females (40.4degrees). Influencing anthropometric values to atd angles in males were distance from triradii a to d, the ratio of the length from triradius t to the DWC to palm length, palm length. Influencing anthropometric values to atd angles in females were the ratio of the length from triradius t to the DWC to palm length, the ratio of the distance from triradii a to d to palm breadth, and the ratio of palm breadth to palm length. In addition to, the regression equation predicted atd angle of males was 26.150+(0.741xdistance from triradii a to d)+(52.856xthe ratio of the length from triradius t to the DWC to palm length)-(0.332xpalm length), on the other hand, that of females was -42.935+(51.657xthe ratio of the length from triradius t to the DWC to palm length)+(63.314xthe ratio of the distance from triradii a to d to palm breadth)+(42.764xthe ratio of palm breadth to palm length). The results of this study suggest that there were differences in influencing anthropometric values to atd angles between males and females in European American. Especially, the ratio of the length from triradius t to DWC to palm length was a very significantly influencing anthropometric value to atd angles to all of males and females.

Characteristics of Fingerprints According to Type of Handedness / 체질인류학회지

Left handedness is known to be related with inheritance and is different from right handedness in brain development and various aspects of perceptive and cognitive performance. Fingerprints are also related with inheritance and do not change for the whole life. In addition, individuals have different fingerprints. The aim of the present study was to identify genetic correlation between fingerprints and handedness by examining how different fingerprints are between left handers and right handers. The study group to imprint fingerprints was composed of 55 left handers and 144 right handers of 1063 college students. The reliability of handedness assessment tool was Cronbach's alpha=0.948. The imprinted fingerprints were classified by fingerprint type (arch, radial loop, ulnar loop, whorl). Finger ridges and triradii in fingerprints were also counted. We performed frequency analysis, reliability analysis, cross-tabs analysis, chi-square test, independent t-test, paired t-test by SPSS win 15.0 for the data. The left handed males and females exhibited more arch types than the right handers and less whorl types than the right handers. The left handers in both hands exhibited more arch and ulnar loop types than the right handers and less whorl and radial loop types than the right handers. In the left hand, the 3rd (p<0.05), 4th (p<0.05), and 5th (p<0.01) fingerprints of the left handers were different from those of the right handers. The finger ridge counts of left handers were significantly less than those of the right handers in the 2nd and 3rd fingers of the left hand, and in the 3rd and 4th fingers in the right hand. The triradii counts of left handers were significantly less than those of right handers (p<0.05) in the 3rd, 4th, and 5th finger of the left and right hand. Total triradii counts of left handers were also significantly less than those of right handers (p<0.05). These results demonstrate that fingerprint type, finger ridge and triradius counts of the left handers are different from those of the right handers, and fingerprints may reflect genetic tendency for handedness.

Relations of Atd Angles and Anthropometric Values of Palms according to Sex Distinction / 체질인류학회지

The palm prints are unique in each individual and permanent for a lifetime. The atd angle of palm is formed by triradii of palm prints. The aim of this study is to identify the reasons why males have smaller atd angles, even with larger palms than females have. This study was done on 379 individuals (males : 174 individuals, females : 205 individuals). Data were collected by measuring atd angles using a protractor, distance from triradii a to d, length from triradii t to the distal palmar crease (DPC), palm breadth at metacarpals and palm length perpendicular using digital callipers. The data were analyzed through the frequency, independent sample t-test, paired t-test, Pearson correlation analysis using SPSS win 14.0. This study showed that atd angles of 39.2 degrees in males are significantly smaller than those of females (40.5 degrees). Males' were wider than females' in palm breadth, palm length and distance from triradii a to d, however, there was no sexual difference in the length from triradii t to the DPC. In addition, the ratio of palm breadth to palm length was higher in males than in females, however, the ratio of the length from triradii t to the DPC to palm length, and the ratio of the distance from triradii a to d to palm breadth of males were lower than those of females. Especially, atd angles in males and females were more correlative with the ratio of the length from triradii t to the DPC to palm length. The results of this study suggest that the differences in the atd angles between males and females arise from the ratio of length from triradii t to the DPC to palm length and the ratio of distance from triradii a to d to palm breadth. In both of the cases, males exhibited lower ratios than females. Especially, the ratio of the length from triradii t to DPC to palm length affected more to the atd angle than any other ratios.

The Type of Handedness and Correlation Analysis of Handedness Assessment Items on University Students in Korea / 체질인류학회지

The left handers are different from the right handers in brain development aspects and various perceptive or cognitive performance. There are known the types of handedness are under social and cultural influence. Moreover, the different races or countries show the different sensitivity to each item in assessment tool. The aim of the this study is to assess type of handedness and choose the best item of assessment tool for Korean. The subjects of this study were 1,063 persons. Data were collected by total 31 items including 20 items through reconstruction of the Oldfield Test, Annett Questionnaire and Croviz-Zener Questionnaire. The reliability of tool is Cronbach's alpha=0.948. The data were analyzed through frequency, reliability analysis, t-test, and Pearson's Correlation analysis by SPSS win 14.0. The 11.5% of total subjects was changed handedness through experience, 62.7% of these subjects changed from left handedness to right or mixed handedness, and 30.5% of these subjects changed handedness from sixth years old or more to below eighth years old. The mean of handedness score between parents and offspring revealed statistically significant difference (father and offspring t=-5.227, p<0.001, mother and offspring t=-4.515, p<0.001). The left handedness increased from the past ratio 4.8% (Kang 1994) to the present study 6.0% (64 persons) by handedness assessment tool. The mean of total items showed significant positive correlations with hammering a nail (r=0.892, p<0.001), gripping knife (r=0.871), using scissors (r=0.847), and writing exhibited the lowest correlation (r=0.563). These results demonstrate that left handers in Korea were increased than the results by previous researchers, and the best items for distinguishing the left handers from the right handers were the hand hammering a nail, gripping knife, using scissors, lighting a match, and throwing small ball.

Distribution and Role of Ovarian Follicle Macrophage in Rat Ovarian Follicular Atresia / 대한산부인과학회지

OBJECTIVE: Ovarian follicular atresia is initiated from ovarian granulosa cell apoptosis and macrophages exert their effects directly and/or indirectly on follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. In spite of the abundant data on ovarian macrophages, the presence of these cells within the follicles (i.e., among granulosa cells) remains controversial and the elimination methods of apoptotic bodies of atretic follicles, and the time and methods of penetration of macrophages into the follicles are not known completely. The aim of the present study is to demonstrate the presence of macrophage within the ovary as related to follicular atresia and the process of elimination of apoptotic granulosa cells by light and electron microscopy. METHODS: Using rat ovaries, immunohistochemical studies with rat macrophage monoclonal antibody ED1 for macrophages, and light and transmission electron microscopic observations were performed. RESULTS: In the rat, follicular atresia was initiated by the granulosa cell apoptosis which occured randomly within the all granulosa layers. Macrophages were observed within normal follicles, in antrum, granulosa and theca cell layers of atretic follicels, in interstium and in corpus luteum. Ultrastructurally, apoptotic granulosa cells showed characteristics, pyknotic nucleus and apoptotic body formation. Apoptotic bodies were eliminated by intact neighboring granulosa cells and macrophages. Intact granulosa cells ingested apoptotic bodies transiently, soon after they fell into the apoptosis. Finally, apoptotic bodies and degenerating oocytes were phagocytosed by macrophages. Macrophages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progression of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia. CONCLUSION: This study demonstrates both intact neighboring granulosa cells and macrophages in the elimination of apoptotic bodies in atretic follicles of the rat ovary. Macrophages are present within normal follicles, in atretic follicles such as antrum, granulosa and theca cell layers and in corpus luteum but are in different appearances according to their location in ovary. A number of macrophages appearing in atretic follicles and in corpora lutea suggest a role for macrophages in follicular atresia and corpus luteum differentiation. The function of macrophage according to their location in follicular development should be further studied.

The Effect of X-irradiation on the Developing Incisor Tooth of the Rat / 체질인류학회지

In the development of tooth, the first sign is the localized thickening of oral ectoderm to form the tooth bud, and then through the dialogue between the bud and underlying mesenchyme, proliferation and differentiation of bud cells and surrounding mesenchymal cells continue, and cap and bell stages follow. In each step of development, various teratogens may act directly and indirectly, and may result a certain congenital anomalies. To reveal the action mechanism of ionizing radiation on odontogenesis morphologically, 4 Gy X-ray irradiated on the rat (Sprague-Dawley strain) fetus on GD 12.7, and observed the histological changes of the upper incisor tooth from GD 13.5 to GD 20.5, daily. In the normal development of upper incisor tooth of rat, the bud stage was from GD 12.5 ~GD 15.5, the cap stage was from GD 16.5 to GD 17.5, and the bell stage was GD 18.5 to GD 20.5. After X-irradiation on GD 12.7, the development of incisor tooth was delayed markedly, the bud stage was prolonged from GD 13.5 to GD 17.5, and the cap stage was GD 18.5 and the bell stage was from GD 19.5 to GD 20.5. After X-irradiation, from GD 13.5 to GD 16.5, apoptosis is observed in the dental organ and surrounding mesenchyme, hemorrhagic necrosis began from GD 16.5 at the center of dental pulp, followed ischemic necrosis of dental organ and surrounding mesenchyme. After GD 19.5, the development of tooth was ceased. These suggest that at least 2 mechanisms involved during X-ray teratogenesis on developing tooth. After X-irradiation on GD 12.7, initially, X-ray induced apoptosis of the cells of dental lamina and dental bud, which resulted the delayed proliferation and differentiation of dental bud and shortage of the number of cells having signal molecules which induce aggregation of the underlying mesenchymal cells. Lately, disorganization of the endothelial cells differentiated from the damaged mesenchymal cells, which resulted in rupture of capillaries and the hemorrhagic necrosis.

Germinal Center Formation and Integrins Expression in the Rat Thymus After 5-Fluorouracil Treatment / 대한해부학회지

Thymus is a lymphoid organ forming T-cells from hematogenous stem cells. There is no report on the germinal centers in the thymus except the Myasthenia gravis in human. In this study, new germinal centers were experimentally induced from 6 days after 5-FU treatment in the Sprague-Dawley rats. With germinal center formation, proteins of molecular weight 123 kDa on 6 days after 5-FU treatment, and 162 kDa and 205 kDa on 9 days after 5-FU treatment were increased in 5-FU treated group. These proteins revealed alpha 1 -and beta 1 -integrins on integrin Western Blot. In this experiment, it was cosidered that alpha 1-integrins and beta 1 -integrins were the key proteins for proliferation of B cells within the newly formed thymic germinal centers and the massive apoptotic disposal of B-cells from the germinal centers, and the new formation of T cells within the cortex.

A Case of Horseshoe Kidney / 체질인류학회지

During cadaver dissection at Chungnam National University in year 2001, we found a case of horseshoe kidney. The characteristic findings of this kidney were as follows; 1. Horseshoe kidney was located at the level of 12th thoracic vertebra and 4th lumbar vertebra, and its isthmus was located at the level of 3rd lumbar vertebra, just below inferior mesenteric artery. The upper pole of the right kidney was 11 mm higherthan that of left kidney. 2. Both renal arteries originated normally from the abdominal aorta below superior mesenteric artery and divided into 2 branches at the front of the renal hilum. The lower branches entered normally into the renal hilum respectively, but, 2 upper branches of right renal artery and 3 upper branches of left renal artery entered into the upper segment of both kidneys respectively. 3. The 2 accessory renal arteries were found. One was the branch of the median sacral artery, which asended anterior to the bifurcation of abdominal aorta and divided 2 branches, of which larger right branch entered inferior pole of right kidney and smaller left branch entered into the isthmus. The other was originated from abdominal aorta 1/3 distance from the origin of inferior mesenteric artery to the bifurcation of abdominal aorta, and entered into the posteroinferior part of left kidney. 4. There were additional 2~3 minor calyces in the lower part of the both kidneys in frontal section, which formed a major calyx draining into the renal pelvis. Parenchymal tissues of both kidneys were continuous through isthmus. In frontal section, renal pyramids were twice in number, and arranged into 2 groups at the upper and lower parts of the both kidneys. Especially, one renal pyramid laid transversely in the isthmus and the renal papilla of it opened into the minor calys of left kidney. It is thought that this horseshoe kidney might be resulted from the elongation of a ureteric bud, which induced new broad -field nephron within the metanephric blastema, and formed a group of additional renal pyramids. The additional renal pyramids formed slight later than normal period, and the separation of both kidneys should be failed and resulted horseshoe kidney. During ascent of kidney, the inferior mesenteric artery interrupted upward migration. It can be concluded that the error of reciprocal induction between ureteric bud and metanephric mesenchyme may be an important mechanism of horseshoe kidney formation.

Histological and Biochemical Study on the Effect of Cadmium Chloride on the Rat Testis / 대한해부학회지

Although cadmium is a well known heavy metal which has an influence testis and brings about male infertility, the mechanism of action in the testis is still fully unknown. In these experiment, cadmium chloride 4 mg/kg of body weight administered intraperitoneally to the rat (Sprague-Dawley) and sacrificed after 1 week, and morphological changes were observed by LM and TEM. In addition, electrophoresis, immunoprecipitation and Western blotting and N-terminal analysis performed to reveal the protein changes. 1. Major findings under light microscope were hemorrhagic necrosis and death of all the spermatogenic cells and supporting cells within the seminiferous tubules, and decreased volume of ECM, many apoptotic bodies, and death of interstitial cells and fibroblasts within interstitium. 2. The EM findings were disruption of nuclear membrane and disappearance of cell organelles of spermatogenic cells and supporting cells within seminiferous tubules, and decreased filopodia, increased inclusion bodies, vacuolation and apoptotic changes of the interstitial cells and fibroblastic cells, many short electron-dense collagen fibers in the extracellular matrix of interstitium. 3. Two proteins of molecular weight 42 kDa and 21 kDa which disappeared after cadmium treatment were rat collagen type I alpha 2. According to the above results, it is considered that cadmium degrades the collagen of the wall of small blood vessels within seminiferous tubules and interstitium and disrupts vascular walls, which results hemorrhagic necrosis, death of all the spermatogenic cells, and the death of interstitial cells and fibroblastic cells.

5-Fluorouracil-Induced Apoptosis in the Rat Thymus / 체질인류학회지

Thymocyte death has been recognized as one of the best characterized experimental models of apoptosis that can be induced by a variety of stimuli such as glucocorticoids, ionizing radiation, anticancer drugs and toxins. The aim of this study was to investigate 5-fluorouracil (5-FU)-induced apoptosis in the rat thymus morphologically. Sprague Dawley rats were injected intramuscularly with 5-FU 100mg/kg body weight and 200mg/kg body weight, and were then sacrificed on day 1, 3, 6, 9 and 12 after treatment. H-E stain and the TUNEL method using the ApopTag kit were used. The results were as follows: 1. On day 1 and 3 after 5-FU treatment, thin cortex and prominent reduction of thymocytes in the cortex were detected. The degree of change was more extensive in the 5-FU 200 mg/kg-treated group than in the 5-FU 100 mg/kg-treated group. Pleomorphic apoptotic cells detected in the cortex were increased in the 5-FU 200mg/kgtreated group and on day 3 after treament. 2. In the 5-FU 100mg/kg-treated group, germinal centers appeared in the cortex on day 6 after treatment starting recovery. Formation of germinal centers continued to day 9 after treatment. In the germinal centers, there were large numbers of typical round apoptotic cells. The thymus exhibited normal architecture on day 12 after treatment. In the 5-FU 200 mg/kg-treated group, however, there were some that did not recover from involution of the thymus on day 6 after treatment and showed fibrosis with decrease of thymocytes and increase of connective tissue to day 9 after treatment. Others recovered and occurred similar changes to the 5-FU 100mg/kgtreated group. 3. 5-FU-induced pleomorphic apoptosis appeared mainly in the cortex and increased gradually depending on dose up to day 1, 3 after treatment. Apoptosis in the germinal centers first occurred from day 6 after treatment and revealed typical large round apoptotic cells. According to the above results, it is thought that 5-FU has an inhibitory effect on DNA synthesis, cell death and involution on surrounding tissue by direct action on thymocytes. The effect continues upto day 3~4 after treatment. Meanwhile, along with the recovery of the thymus from day 6 after treatment, new germinal centers appeared and active lymphocytes production occurred in the germinal centers, which was considered as compensatory reaction. To eliminate enormous B cells newly produced in the germinal center, active apoptosis would occur.

Morphogenesis of the Hindlimb Digits and 5-FU induced Apoptotic Changes / 체질인류학회지

During the morphogenesis of the hindlimb and the digits of the rat, apoptosis has a crucial role to make their normal shape. In this study, apoptotic changes of the hindlimb from GD 13.5 to GD 17.5 were observed and compared with 5 -FU treated group to reveal the mechanism of teratogenic action of 5 -FU. The results were as follows; 1. The mean body weight of rat fetuses was decreased significantly in the 5 -FU treated group. 2. Congenital anomalies of the hindlimb digits were cleft foot, syndactyly and short digits, and the occurance rate of congenital anomalies was 58.1%, in which right -sided anomalies was 49.1% and left -sided anomalies was 18.9%. 3. Normal morphogenesis of the hindlimb digits was as follows; initially, hindlimb bud appeared at the lateral wall of lumbar somite on GD 10.5, grown as an elliptical mass proximodistally on GD 13.5, flattening and digital rays formation on GD 14.5, interdigital notches appeared on GD 15.5, interdigital spaces formation and interphalageal joints formation on GD 16.5, and deepening of interdigital spaces and elongation of digits on GD 17.5. 4. 5 -FU inhibited differentiation and growth of hindlimb bud initially, but its effect on development of hindlimb was disappeared after GD 15.5. According to the above results, it is considered that 5 -FU has an inhibitory effect on differentiation and growth of hindlimb of rat fetuses, and the TUNEL immunohistochemical method is much available to detect normal morphogenesis of an organ and to detect the mechanism of action of various teratogens.

Two Cases of Sternalis of Korean / 체질인류학회지

No abstract available.

Analysis of FUDR --induced Congenital Anomalies in the Rat / 체질인류학회지

For the study on the teratogenic effects and its mechanism of FUDR on the developing rat fetuses, Sprague -Dawley rats as experimental animal and 5 -fluoro -2 '-deoxyuridine (Sigma chemicals) as FUDR were used respectively. On the day of gestation 10.5, 60 mg/kg, 65 mg/kg and 70 mg/kg of FUDR was injected intraperitoneally, and sacrificed under ether anesthesia on the day of gestation 17.5. External congenital malformations such as hydrocephalus, lens defect, cleft palate, short tail, forelimb and hindlimb malformations were observed under stereoscope, and compared each other with control group. The results were as follows; 1. Congenital anomalies induced by FUDR were hydrocephalus, lens defect, cleft palate, short tail, and defects of extremities such as phocomelia, loss of first and second digits, undergrowth of digits, and syndactyly. 2. There is close relation between the rate of occurance of congenital anomalies and doses of FUDR in some organs. 3. There is significant differences between forelimbs and hindlimbs and between right forelimb and left forelimb in the rate of occurance of congenital anomalies of the extremities. According to the above results, it is considered that there is significant differences between the types and occurance rates of congenital anomalies induced by FUDR and the doses of FUDR. But distinct mechanism of action during teratogenesis of FUDR still remains unclear.